Autophagy induction in Jurkats measured by flow cytometry using Autophagy Probe, Red

Jurkat cells were either untreated for basal conditions (red), treated with 0.5 μM Rapamycin for 18 hours (blue), or treated with 0.5 μM Rapamycin for 18 hours and 10 nM Bafilomycin A for 2 hours (orange) to induce autophagy. After staining with Autophagy Probe, Red for 30 minutes, cells were washed and analyzed on the ZE5 Cell Analyzer using the 561 nm laser and 640/20 filter

Autophagy induction in Jurkats measured by flow cytometry using Autophagy Probe, Red

Jurkat cells were either untreated for basal conditions (red), treated with 0.5 μM Rapamycin for 18 hours (blue), or treated with 0.5 μM Rapamycin for 18 hours and 10 nM Bafilomycin A for 2 hours (orange) to induce autophagy. After staining with Autophagy Probe, Red for 30 minutes, cells were washed and analyzed on the ZE5 Cell Analyzer using the 561 nm laser and 640/20 filter